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  • Title: A simplified method for determining undeclared sweeteners added to pure orange juice.
    Author: Iuliano TA.
    Journal: J AOAC Int; 1996; 79(6):1381-7. PubMed ID: 8946716.
    Abstract:
    Previous research has described methods for monitoring unique oligosaccharides as indicators of adulteration of pure orange juice with undeclared sweeteners. Modifications are reported here that streamline the method for routine analysis of large numbers of samples. Sample preparation is faster and more efficient, allowing preparation of up to 24 samples at one time. Oligosaccharides are eluted isocratically at 30 degrees C, decreasing retention times and eliminating the need for column reequilibration between injections. Oligosaccharide profiles of commercially available sweeteners and fortified orange juice samples analyzed under the modified conditions demonstrate that the method reduces analysis time by > 50%, while at the same time improving resolution and maintaining established detection limits.
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