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  • Title: Capture in the gel: intermoleculare triplex formation during gel electrophoresis.
    Author: Belotserkovskii BP, Johnston BH.
    Journal: Electrophoresis; 1996 Oct; 17(10):1528-34. PubMed ID: 8957174.
    Abstract:
    Analysis of unusual gel mobility patterns formed by certain DNA triplexes has revealed that intermolecular triplex formation can occur during gel electrophoresis when a faster migrating single strand overtakes a slower migrating band containing a duplex of appropriate sequence. Control experiments showed that this capture of the third strand occurs by sequence-specific hybridization rather than some nonspecific retardation. This phenomenon can be used to detect triplexes by a gel-shift assay even if their lifetime is much shorter than the time of gel electrophoresis.
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