These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: CD7-negative T cells represent a separate differentiation pathway in a subset of post-thymic helper T cells.
    Author: Reinhold U, Liu L, Sesterhenn J, Abken H.
    Journal: Immunology; 1996 Nov; 89(3):391-6. PubMed ID: 8958052.
    Abstract:
    The absence of CD7 protein and the corresponding mRNA is a stable feature in a subset of normal circulating CD4+ memory T cells. It is still unresolved whether the CD7- subset represents a specific T-cell lineage. Here we show that repeated stimulation of highly purified CD4+ CD45RA+ CD45RO- naive T cells in vitro leads to the development of a distinct memory subset that is defined by the expression versus non-expression of the CD7 antigen. Comparing different T-cell activation pathways (TCR/CD3, CD2), we observed that alternative signals were critically involved in the development of CD4+ CD7- T cells. Peak mean numbers of CD7- memory cells occurred after 3-5 cycles of restimulation in vitro. Naive T cells that had undergone repeated stimulations were harvested and sorted into CD7+ and CD7- subsets. The vast majority (> 97%) of CD7+ T cells retained their expression, whereas the CD7- population did not re-express the antigen during further propagation of separated T-cell subsets. In CD7- cells no CD7 mRNA was monitored, indicating transcriptional regulation of CD7 expression. Certain differentiation-related antigens, including the cutaneous lymphocyte antigen CLA, were preferentially expressed on CD7- T cells. We suggest that absence of CD7 expression in a subset of CD4+ memory cells reflects a separate and stable differentiation state occurring late in the immune response. These T cells may represent the physiological counterpart of malignant T cells in certain forms of cutaneous T-cell lymphoma.
    [Abstract] [Full Text] [Related] [New Search]