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  • Title: [ELISA in the rapid diagnosis of influenza using as the detecting antibodies polyclonal antinucleoprotein sera].
    Author: Lupulescu E, Ioniţă E, Constantinescu C, Tecu C, Chiriţă C, Alexandrescu V.
    Journal: Bacteriol Virusol Parazitol Epidemiol; 1996; 41(1-2):63-7. PubMed ID: 8963120.
    Abstract:
    Hyperimmune polyclonal sera were obtained in order to use them as capture and detecting antibodies in rapid diagnostic of influenza A and B viruses by ELISA. The test shown 100% specificity. The sensitivity in directly detection of influenza viruses in human samples compared of influenza isolations was 49,25% for type A and 53,33% for type B. The sensitivity was increased after 48 hours from inoculation of human samples in cell culture-86,75% for type A detection and 80,0% for type B detection from supernatant mammalian tissue culture (MDCK).
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