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  • Title: Conversion of GC-->AT recognition and elucidation of AT recognition mechanism in zinc finger transcription factor by permutational approach.
    Author: Emori T, Nagaoka M, Sugiura Y.
    Journal: Nucleic Acids Symp Ser; 1995; (34):3-4. PubMed ID: 8966119.
    Abstract:
    Zinc finger-DNA recognition has been investigated through GC-->AT conversion of recognition sequence 5'-GGG-GCG-GGG-3' of transcription factor Sp1 containing three zinc fingers. Three critical amino acid residues of the finger 2 in Sp1 were replaced with the corresponding residues of the finger 4 in CF2-II. The finger 2 of the hybrid protein is expected to recognize 5'-ATA-3' sequence. Indeed, the designed protein Sp1QTQ2 specifically bound to ATA and ATAN probes. The present study demonstrates that one zinc finger recognizes total sequence of the target DNA site rather than triplet base sequence.
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