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  • Title: Structure and analysis of phospholipase D gene from Ricinus communis L.
    Author: Xu L, Zheng L, Coughlan SJ, Wang X.
    Journal: Plant Mol Biol; 1996 Nov; 32(4):767-71. PubMed ID: 8980529.
    Abstract:
    Phospholipase D (PLD; EC 3.1.4.4) has been proposed to play a pivotal role in various cellular processes, but molecular understanding of this enzyme is rather limited. This report describes the nucleotide sequence, structure, and genomic organization of a PLD gene from castor bean (Ricinus communis L. cv. Hale). The PLD gene was isolated from a castor bean genomic library using the PLD cDNA as a hybridization probe. Sequence comparison with the PLD cDNA revealed that the PLD gene consisted of four exons and three introns, one of which interrupts the 5'-untranslated region. Southern blot analysis indicated that the cloned PLD gene was present as a single-copy gene, and yet there were other PLD or PLD-related sequences in the castor bean genome.
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