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  • Title: [Effect of cryoprotectants on protein-synthesizing activity of cell-free system from rat liver].
    Author: Gulevs'kiĭ OK.
    Journal: Ukr Biokhim Zh; 1977; 49(4):39-42. PubMed ID: 898316.
    Abstract:
    The protein-synthetizing activity in postmitochondrial supernatant (S15) was studied as affected by ethylene glycol, PEG-400, PEG-4000, DMSO, ethanol, methanol, and glycerol. It is found that cryoprotectants inhibit 14C-leucine incorporation in the presence of physiological concentrations of Mg2+ (5 mM) in the cell-free system. All cryoprotectants, exept of glycerol, shift the maximum of 14C-leucine incorporation towards lower Mg2+ concentrations in the cell-free system.
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