These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Polymer-shielded dye-ligand chromatography of lactate dehydrogenase from porcine muscle in an expanded bed system.
    Author: Garg N, Galaev IYu, Mattiasson B.
    Journal: Bioseparation; 1996 Jun; 6(3):193-9. PubMed ID: 8987685.
    Abstract:
    Dye-affinity chromatography is a widely used technique in protein purification. It has recently been shown that the efficiency of the chromatography process can be significantly improved by pretreatment of the affinity matrix with certain water soluble polymers such as poly(vinyl pyrrolidone). This technique termed as polymer-shielded, dye-affinity chromatography has been successfully used in packed bed mode at a lab scale for the purification of a number of enzymes. The present work deals with the application of polymer-shielded dye-affinity chromatography in an expanded bed system of Streamline-Cibacron Blue 3GA for the isolation of lactate dehydrogenase from a crude porcine muscle extract. The elution conditions were optimised to obtain an efficient process. A higher recovery of the target enzyme (78%) was obtained from the polymer shielded column as compared to the unshielded column (17%), after low ionic strength elution. The purification factor obtained after chromatography on the polymer shielded column was higher (4.1) than that from unshielded column (1.8).
    [Abstract] [Full Text] [Related] [New Search]