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  • Title: [Comparative study of the effect of fatty acids and certain spin effectors on quaternary structure and spin state of methemoglobin].
    Author: Bovdeĭ NA, Kiseleva SN, Kiselev PA.
    Journal: Biokhimiia; 1996 Sep; 61(9):1570-7. PubMed ID: 8998281.
    Abstract:
    Cysteine beta-93 SH-groups of horse and bovine methemoglobins (metHb) were modified by N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide. Effects of pH, F-, CN-, oleic acid, and sodium dodecyl sulfate on spin and conformational dynamics of the protein were studied by electron and EPR spectroscopy. Oleic acid and CN- had similar effects on both parameters. Relationship between spin and conformational transformations of metHb was elucidated. Effect of oleic acid can be due to increased conformational mobility of C-terminus of metHb beta-chains.
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