These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Determination of penicillin-V in human plasma by high-performance liquid chromatography and solid-phase extraction.
    Author: Krauwinkel WJ, Volkers-Kamermans NJ.
    Journal: J Chromatogr B Biomed Appl; 1996 Apr 26; 679(1-2):129-35. PubMed ID: 8998551.
    Abstract:
    A high-performance liquid chromatographic method has been developed for the determination of penicillin-V concentrations between 0.1 and 19 micrograms/ml in human plasma. Penicillin-V was isolated from plasma by solid-phase extraction on a C18/OH cartridge. The extracts were injected onto a reversed-phase HPLC system. A 125 x 4 mm C18 column was used to separate penicillin-V from its main metabolites, 5R- and 5S-penicilloic acid and endogenous compounds. The eluent consisted of 66% 0.02 M phosphoric acid buffer, to which tetrabutylammonium dihydrogenphosphate and 34% acetonitrile were added. The column effluent was monitored by ultraviolet spectrophotometry at 269 nm. Using this method, penicillin-V concentrations in plasma could be determined with an accuracy between -5.4 and 5.2% and a precision between 0.8 and 1.6%. The method has proved to be reliable and was used in bioavailability studies for the development of a new oral penicillin-V formulation.
    [Abstract] [Full Text] [Related] [New Search]