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  • Title: Routine determination of flumequine in kidney tissue of pig using automated liquid chromatography.
    Author: Guyonnet J, Pacaud M, Richard M, Doisi A, Spavone F, Hellings P.
    Journal: J Chromatogr B Biomed Appl; 1996 Apr 26; 679(1-2):177-84. PubMed ID: 8998558.
    Abstract:
    A high-performance liquid chromatographic assay is described as a routine analytical method for the determination of flumequine (FLU) and its hydroxylated metabolite (OH-FLU) in pig kidney tissue. Kidney samples (2 g) containing FLU and OH-FLU were extracted by liquid-liquid extraction with ethyl acetate (10 ml). Analytical separations were performed by reversed-phase HPLC with fluorometric detection at 252 nm excitation and 356 nm emission under gradient conditions. The mobile phase was acetonitrile-2.7.10(-3) M oxalic acid in water (pH 2.5). The assay is specific and reproducible within the flumequine range of 0.050-2.5 micrograms/g and recovery at 0.050 microgram/g was 94.8%.
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