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  • Title: [Thin-layer chromatography analysis of methamphetamine in urine samples].
    Author: Klimes J, Pilarová P.
    Journal: Ceska Slov Farm; 1996 Nov; 45(6):279-83. PubMed ID: 8998609.
    Abstract:
    The paper investigated the chromatographic conditions for TLC analysis of metamphetamine on silica gel and the lipophilic stationary phase RP V 18 in order to qualitatively analyze the drug in urine samples. Attention was also paid to chemical detection. Five detection reagents were tested, out of which Fast Black K salt, yielding orange-red spots with a detection limit of 1 microgram, proved to be the best. In the analysis of metamphetamine in the samples of model urine, the best results were achieved on Kieselgel. Using the developing system ethyl acetate-ethanol-concentrated solution of ammonia (36:2:2), a complete separation of the metamphetamine spot from the spots of ballast from the biological matrix was achieved.
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