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  • Title: PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures.
    Author: Valente P, Gouveia FC, de Lemos GA, Pimentel D, van Elsas JD, Mendonça-Hagler LC, Hagler AN.
    Journal: FEMS Microbiol Lett; 1996 Apr 01; 137(2-3):253-6. PubMed ID: 8998994.
    Abstract:
    The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.
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