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  • Title: Isolation and characterization of diploid clones from adult and newborn rat liver cell lines.
    Author: Tokiwa T, Nakabayashi H, Miyazaki M, Sato J.
    Journal: In Vitro; 1979 Jun; 15(6):393-400. PubMed ID: 90011.
    Abstract:
    A high frequency of diploid and near-diploid clones were developed from cell lines derived from adult and newborn rat liver using micropipettes. There were some differences in morphology, biochemical properties and growth rate between clones. Cloned cells had low levels of tyrosine transaminase activity, glucose-6-phosphatase activity and albumin content. A diploid clone and pseudodiploid clone derived from adult rat liver cell line were positive for alpha-fetoprotein.
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