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  • Title: An investigation into the immunochemical properties of the isoenzymes of human alkaline phosphatase.
    Author: Abdullah JS, Gowland E.
    Journal: Ann Clin Biochem; 1977 Jul; 14(4):212-7. PubMed ID: 900854.
    Abstract:
    A study was carried out to investigate the immunochemical properties of the alkaline phosphatases present in liver, placenta, intestine, and kidney. Butanol extracts of the tissues were examined by antigen-antibody crossed electrophoresis, and the immunoprecipitates formed by the enzymes were identified by use of a specific stain for alkaline phosphatase. The results obtained revealed three immuno-chemical types of alkaline phosphatase, designated L, P, and PI respectively. The distribution of these enzymes among the tissues examined was as follows: type L was the only enzyme detected in liver extract and type P the only one detected in placental extract. The extracts of intestine and kidney both contained two isoenzymes, one of which was type L and the other type PI.
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