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  • Title: Evaluation of HBsAg carrier rate in acute viral hepatitis and high risk individuals using RPHA and ELISA.
    Author: Korgaonkar A, Verenkar MP, Savio R, Pinto MJ, Singh I.
    Journal: Indian J Pathol Microbiol; 1996 Oct; 39(4):277-80. PubMed ID: 9009478.
    Abstract:
    A total of 600 individuals including 500 cases of hepatitis and 100 individuals at high risk for developing hepatitis were screened for hepatitis B surface antigen (HBsAg) using reverse passive haemagglutination (RPHA) and enzyme linked immunosorbent assay (ELISA). HBsAg carrier rate in clinically diagnosed cases of hepatitis was 38% and 32.4% by RPHA and ELISA respectively. In high risk individuals, the carrier rate was 14% by RPHA and 11% by ELISA. Taking ELISA as gold standard, RPHA showed 5.33% false positivity and 0.33% false negativity. The over all correlation between RPHA and ELISA was to the tune of 82.66%.
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