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  • Title: Effect of lipid extraction methods on total dietary fiber and nonstarch polysaccharide contents of selected nuts and seeds.
    Author: Li BW, Zhao Z, Jekot JJ.
    Journal: J AOAC Int; 1997; 80(1):98-101. PubMed ID: 9011063.
    Abstract:
    Three extraction methods were used to remove lipid materials from 8 edible nuts and seeds before analysis for their total dietary fiber (TDF) and nonstarch polysaccharide (NSP) contents. Portions of ground materials were extracted by: n-hexane, followed by 80% methanol, n-hexane-acetic acid (95 + 5, v/v), and supercritical carbon dioxide. Defatted samples were gelatinized in water and incubated with amyloglucosidase; 95% ethanol was added to the hydrolyzates, and the residues were collected on tared glass crucibles. TDF was calculated according to a simplified enzymatic-gravimetric method developed in our laboratory, and NSP was determined as described by Englyst and coworkers. Dietary fiber values obtained with any of the extraction methods range from 3.47 g/100 g for cashews to 28.56 g/100 g for sunflower seeds. Nonstarch polysaccharide values range from 2.43 g/100 g for pecans to 5.56 g/100 g for peanuts. With the exception of sunflower seeds, samples extracted with supercritical carbon dioxide had lower TDF contents but similar amounts of NSP compared with samples extracted by the other 2 methods.
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