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  • Title: "Immunosubtraction" electrophoresis: a simple method for identifying specific proteins producing the cellulose acetate electrophoretogram.
    Author: Aguzzi F, Poggi N.
    Journal: Boll Ist Sieroter Milan; 1977 Jul 31; 56(3):212-6. PubMed ID: 901635.
    Abstract:
    To identify the proteins that are responsible for the electrophoretic pattern on cellulose acetate, we propose a method based on the precipitation of various individual proteins at the beginning of the electrophoretic run. Any non antibody proteins in the monospecific antisera were removed with a preliminary electrophoretic fractionation of the antiserum. Using this method, we could remove a band or a zone from the pattern, leaving the rest of the pattern unaltered. This permitted us not only to locate a protein in the pattern, but also to better interpret the electrophoretogram. The method requires no special apparatus and it is fast and cheap.
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