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  • Title: Does calcitonin gene-related peptide act as a chemoattractant for rat gubernacular cells?
    Author: Sugita Y, Paxton GA, Hasthorpe S, Hutson JM.
    Journal: J Pediatr Surg; 1997 Jan; 32(1):15-7. PubMed ID: 9021558.
    Abstract:
    Calcitonin gene-related peptide (CGRP) receptors are located in the cremaster muscle of the gubernaculum of rats, and causes gubernacular contraction in vitro, suggesting that CGRP plays an important role in testicular descent. It has been postulated that CGRP released from the genitofemoral nerve acts as a "chemoattractant" for gubernacular migration to the scrotum. The aim of this study is to investigate whether the gubernacular cells of rats exhibit a chemotactic response to CGRP in vitro. Chemotaxis of gubernacular cells from Sprague-Dawley rats (1 to 6 days old) was measured using blind-well chambers. The migration of cells, which passed from the upper to the lower compartment through a polycarbonate filter, were counted using microscopy. The lower compartment included 10(-11), 10(-10), 10(-9), and 10(-8) M CGRP or phosphate buffered saline (PBS) as control. The chemotactic index (CI) was defined as the ratio of migration toward CGRP versus PBS as control. There was no significant migration even at varying CGRP concentrations. Isolated rat gubernacular cells therefore did not exhibit a chemotactic response to CGRP and the role CGRP plays in testicular descent still remains unclear. This result does not exclude the possibility that the gubernaculum responds to CGRP as a whole organ rather than as individual cells.
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