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  • Title: Selectivity of the human sperm-zona pellucida binding process to sperm head morphometry.
    Author: Garrett C, Liu DY, Baker HW.
    Journal: Fertil Steril; 1997 Feb; 67(2):362-71. PubMed ID: 9022616.
    Abstract:
    OBJECTIVE: To obtain quantitative measures of morphometric selectivity of the human spermzona pellucida binding process as determined by light microscopy. DESIGN: Fully automated sperm head morphometric based on a 32-dimensional parameterization of images of Shorr-stained sperm. Zona pellucida selected sperm removed from reinseminated oocytes that previously failed IVF. SETTING: Academic research group associated with a tertiary infertility service. PATIENT(S): Semen samples from 51 infertile patients. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Differences in morphometric parameter means observed before and after swim-up and binding to zonae pellucidae. RESULT(S): Significant differences between insemination and bound sperm were observed in 17 parameter means and 21 standard deviations. The sperm-zona pellucida binding process preferentially selects sperm heads with a large anterior region with relatively low optical density, as well as high axial symmetry and minimal neck anomalies. Bias against sperm with pyriform morphology was not observed. CONCLUSION(S): A causal link has been established between sperm head morphometry, particularly within the acrosomal region, and the ability of sperm to bind to the human zona pellucida. As sperm-zona binding is necessary for fertility, it is possible to derive a physiologically based assessment for clinical diagnosis of male infertility using the "zona-preferred" morphometry results.
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