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  • Title: Identification of the gene encoding DNA topoisomerase I from Candida albicans.
    Author: Taylor A, Giles K, Sarthy AV, McGonigal T, Fostel J.
    Journal: FEMS Microbiol Lett; 1996 May 01; 138(2-3):113-21. PubMed ID: 9026437.
    Abstract:
    A gene encoding a type I topoisomerase (TOP1) was isolated from Candida albicans, sequenced, and expressed in Saccharomyces cerevisiae. The TOP1 gene was identified from a C. albicans genomic library by hybridization with the product of a polymerase chain reaction with degenerate primer sets encoding regions conserved in other TOP1 genes. A clone containing an open reading frame of 2463 bp and predicted to encode a protein of 778 amino acids with sequence similarity to eukaryotic type I topoisomerases was identified. The C. albicans TOP1 gene restored camptothecin sensitivity and increased the topoisomerase activity in S. cerevisiae, indicating that the DNA fragment encodes a functional C. albicans topoisomerase I.
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