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  • Title: Isolation and characterization of chitinase from a flake-chitin degrading marine bacterium, Aeromonas hydrophila H-2330.
    Author: Hiraga K, Shou L, Kitazawa M, Takahashi S, Shimada M, Sato R, Oda K.
    Journal: Biosci Biotechnol Biochem; 1997 Jan; 61(1):174-6. PubMed ID: 9028048.
    Abstract:
    A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an beta-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified and characterized. The optimum pH of Chi-A was 5-8, and the activity was inhibited by Hg2+ and Fe3+. Chi-A was different from chitinases of other Aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. The amino-terminal amino acid sequence showed extensive similarity with chitinases from Gram-negative bacteria.
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