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  • Title: A monolayer coagglutination microplate technique for typing red blood cells.
    Author: Llopis F, Carbonell-Uberos F, Planelles MD, Montero M, Puig N, Atienza T, Alba E, Montoro JA.
    Journal: Vox Sang; 1997; 72(1):26-30. PubMed ID: 9031497.
    Abstract:
    BACKGROUND AND OBJECTIVES: Serologic agglutination tests have the disadvantage of lack of an objective endpoint that can be easily read and quantitated. We have developed a new method for ABO and Rh typing based on producing a red blood cell (RBC) monolayer on microplates and the mixed hemagglutination reaction. MATERIALS AND METHODS: We have employed a new red cell fixation buffer to prepare the RBC monolayer. As the technique for grouping, we used a mixed agglutination reaction between the RBC monolayer and the RBCs to be typed. RESULTS: Compared with an automated hemagglutination system in 34,519 samples, the method is shown to be more sensitive, free of false positive reactions, and cost-effective. CONCLUSIONS: The microplate coagglutination method is accurate and lower in cost than conventional methods.
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