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  • Title: Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections.
    Author: Higuchi S, Suga M, Dannenberg AM, Schofield BH.
    Journal: Stain Technol; 1979 Jan; 54(1):5-12. PubMed ID: 90411.
    Abstract:
    Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, beta-galactosidase, and cytochrome oxidase in plastic embedded and ruotine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 micrometers, were far superior to frozen sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.
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