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  • Title: Direct enantiomeric separation of cathinone and one major metabolite on cellobiohydrolase (CBH-I) chiral stationary phase.
    Author: Aboul-Enein HY, Serignese V.
    Journal: Biomed Chromatogr; 1997; 11(1):47-9. PubMed ID: 9051217.
    Abstract:
    A direct, isocratic high-performance liquid chromatographic (HPLC) method is described for the enantiomeric separation of racemic cathinone and one of its metabolites (+/-)-norephedrine, on a protein-based chiral stationary phase (CSP), cellobiohydrolase, known as CBH-I, under reversed-phase conditions. It was determined that the S-(-)-enantiomer eluted first for cathinone while the 1S,2R-(+)-enantiomer eluted first for norephedrine. The procedure is simple with no sample derivatization required and a short assay time.
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