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  • Title: Application of polymerase chain reaction (PCR) for diagnosis of equine herpes virus-1 (EHV-1).
    Author: Gupta AK, Singh BK, Yadav MP.
    Journal: Indian J Exp Biol; 1996 Nov; 34(11):1077-80. PubMed ID: 9055627.
    Abstract:
    Fifty aborted foetus samples were diagnosed for the presence of equine herpes virus-1 (EHV-1) by polymerase chain reaction (PCR) technique. Specific primer pair for amplification of a particular segment of EHV-1 DNA in gc region having 3 Hae III restriction endonuclease sites was used. A 409 base pair segment obtained as PCR amplification product in 9 samples was digested with Hae III to confirm the presence of EHV-1 as the infectious agent in aborted tissues. It was observed that PCR technique was more sensitive, specific and rapid than the conventional virological diagnostic methods.
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