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  • Title: Application of capillary gas chromatography to the study of hydrolysis of the nerve agent VX in rat plasma.
    Author: Bonierbale E, Debordes L, Coppet L.
    Journal: J Chromatogr B Biomed Sci Appl; 1997 Jan 24; 688(2):255-64. PubMed ID: 9061463.
    Abstract:
    We present here a gas chromatography technique allowing the detection and quantification of VX [O-ethyl S-(2-diisopropylaminoethyl)methylphosphonothiolate] as well as its P-S bond hydrolysis product diisopropylaminoethanethiol directly from spiked rat plasma. This technique was applied to study VX hydrolysis in rat plasma. We observed that 53 +/- 4% of 374 microM VX disappeared from spiked plasma after 2 h. VX disappearance was mainly related to enzymatic cleavage of the P-S bond (Km = 2.5 mM and Vmax = 13.3 nmol min-1 ml-1 of rat plasma). The activity was totally inhibited by 1 mM Hg2+ and was also inhibited by metal chelators.
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