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  • Title: Oronasal leishmaniasis caused by a parasite with an unusual isoenzyme profile.
    Author: Ibrahim M, Suliman A, Hashim FA, Khalil el-T A, Evans DA, Kharazmi A, el Hassan AM.
    Journal: Am J Trop Med Hyg; 1997 Jan; 56(1):96-8. PubMed ID: 9063369.
    Abstract:
    A 45-year-old Sudanese man from western Sudan presented with oronasal leishmaniasis of three years duration. He had no history of previous kala-azar or cutaneous leishmaniasis. The parasite isolated from the oral mucosa was characterized by isoenzymes using 12 enzymes and by polymerase chain reaction amplification of kinetoplast DNA using species-specific primers. The specific primers gave products indistinguishable from those of the Leishmania donovani complex. However, the isoenzyme profile showed a zymodeme pattern that was significantly different from the zymodemes previously reported in the Sudan and the Ethiopian region.
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