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  • Title: Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin.
    Author: Bilić N, Casey M, Blanc B.
    Journal: Biochem J; 1976 Jul 01; 157(1):233-5. PubMed ID: 9066.
    Abstract:
    Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.
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