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Title: Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine. Author: Huerta-Zepeda A, Ortuño L, Du Pont G, Durán S, Lloret A, Merchant-Larios H, Calderón J. Journal: J Bacteriol; 1997 Mar; 179(6):2068-72. PubMed ID: 9068657. Abstract: Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of mutants were obtained: AHZ1, with very low levels of aspartase activity, and AHZ7, with low levels of asparaginase and very low levels of aspartase compared to the wild-type strain. R. etli had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, and modes of regulation. The AHZ mutants nodulated as did the wild-type strain and had nitrogenase levels similar to that of the wild-type strain.[Abstract] [Full Text] [Related] [New Search]