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  • Title: ABO genotyping by PCR-direct sequencing.
    Author: Nata M, Kanetake J, Adachi N, Hashiyada M, Aoki Y, Sagisaka K.
    Journal: Nihon Hoigaku Zasshi; 1997 Feb; 51(1):1-5. PubMed ID: 9078834.
    Abstract:
    The PCR-direct sequence method was applied to ABO genotyping. At the 261st nucleotide of the genes of A and B glycosyltrasferase, it was easily detected that the nucleotide was guanine in AA, AB and BB genotypes and that the nucleotide was ademine in only OO. In AO and BO, substitution of A to G was confirmed by the dye primer method, but it was difficult to detect correctly by the dye terminator method. At the 297th, nucleotide substitution between A and B alleles was confirmed by the both methods. As this position, O allele was subdivided into three types, OAOA, OGOG and OAOG. At the 703rd, nucleotide substitution between A and B alleles was easily detected by the both methods. The PCR-direct sequence method was suitable to confirm the nucleotide substitution or deletion directly and to prevent the mistyping by other methods.
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