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  • Title: Growth and bacteriocin production by Enterococcus faecium DPC1146 in batch and continuous culture.
    Author: Parente E, Brienza C, Ricciardi A, Addario G.
    Journal: J Ind Microbiol Biotechnol; 1997 Jan; 18(1):62-7. PubMed ID: 9079290.
    Abstract:
    Production of the bacteriocin enterocin 1146 (E1146) by Enterococcus faecium DPC1146 was studied in batch and continuous fermentation. Growth was strongly inhibited by lactic acid. In batch fermentations maximum E1146 activity (2.8 MBU L-1) was obtained in 9 h with 20 g L-1 glucose. Increase in initial glucose concentration did not lead to a proportional increase in E1146 activity. A simple linear model was found to be adequate to explain the relationship between specific bacteriocin production rate and specific growth rate in batch fermentations with initial glucose concentration higher than 20 g L-1. Maximum bacteriocin activity (2.9-3.2 MBU L-1) was obtained in continuous fermentations at dilution rates between 0.12 and 0.17 h-1 and specific bacteriocin production rate increased linearly with dilution rate.
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