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  • Title: A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.
    Author: Jäger W, Kalinowski J, Pühler A.
    Journal: J Bacteriol; 1997 Apr; 179(7):2449-51. PubMed ID: 9079937.
    Abstract:
    A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.
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