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  • Title: Application of a simple gas chromatographic technique for measuring breath hydrogen.
    Author: Solomons NW, Viteri FE, Hamilton LH.
    Journal: J Lab Clin Med; 1977 Nov; 90(5):856-62. PubMed ID: 908873.
    Abstract:
    A simple gas chromatographic technique for analyzing H2 in expired air with a compact, relatively inexpensive gas chromatography specifically adapted for H2 analysis is described. Modifications in the basic chromatograph included increasing the capacity of the sample loop, lengthening the column, and using argon as the carrier gas to increase the sensitivity of the system. Methods for collecting and storing respiratory gas samples and for quantifying the results are described for clinical studies of carbohydrate malabsorption. Small errors in collection and quantitation are introduced by the method, but they are minor in relation to the changes in H2 concentration which are seen with significant malabsorption. This simplification of chromatograph technology could increase the general availability of this convenient, noninvasive and well-tolerated test of intestinal absorption of carbohydrates such as lactose.
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