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  • Title: A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution.
    Author: Bruick RK, Koppitz M, Joyce GF, Orgel LE.
    Journal: Nucleic Acids Res; 1997 Mar 15; 25(6):1309-10. PubMed ID: 9092646.
    Abstract:
    A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino- 5'-deoxythymidine in the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5'terminus.
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