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  • Title: Purification of cDNA complementary to sea urchin histone mRNA.
    Author: Woods D, Fitschen W.
    Journal: Nucleic Acids Res; 1977 Sep; 4(9):3187-98. PubMed ID: 909803.
    Abstract:
    Complementary DNA (cDNA) was transcribed from a polyadenylated sea urchin histone mRNA preparation isolated by density gradient centrifugation. By hybridization, this cDNA was shown to be extensively contaminated (85% of hybridizable cDNA) with DNA complementary to RNA derived from the large ribosomal subunit. Purification of a mRNA specific cDNA fraction was achieved by hybridization of purified rRNA to cDNA followed by fractionation on hydroxyapatite. After further purification to remove nonhybridizable cDNA our purified cDNA showed only 8% hybirdization to rRNA.
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