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  • Title: Reversibility of monocytic differentiation of HL-60 cells by 1alpha,25 dihydroxyvitamin D3.
    Author: Tomoyasu S, Fukuchi K, Watanabe K, Ueno H, Hamano Y, Hisatake J, Hino K, Gomi K, Tsuruoka N.
    Journal: Leuk Res; 1997 Mar; 21(3):217-24. PubMed ID: 9111166.
    Abstract:
    Although 1alpha,25(OH)2D3 induces HL-60 cells to differentiate into monocytes, whether or not these monocytes revert to native promyelocytes is not clear. To investigate this question, HL-60 cells were treated with 1alpha,25(OH)2D3, and divided into CD14-positive and -negative cells with a cell sorter. These two populations were cultured with or without 1alpha,25(OH)2D3. Sorted CD14-positive HL-60 cells, treated with 1alpha,25(OH)2D3 for 7 days, reverted to CD14-negative cells and promyelocyte-like cells if 1alpha,25(OH)2D3 was removed from the medium. We conclude that the 1alpha,25(OH)2D3-induced differentiation of HL-60 cells into monocytes is reversible, and continuous administration of 1alpha,25(OH)2D3 is required for the differentiation of HL-60 cells.
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