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  • Title: Influence of the energetic state of rat liver mitochondria on the sensitivity of the phosphate carrier towards SH reagents.
    Author: Le Quoc D, Le Quoc K, Gaudemer Y.
    Journal: Biochim Biophys Acta; 1977 Oct 12; 462(1):131-40. PubMed ID: 911819.
    Abstract:
    Phosphate transport into rat liver mitochondria was measured by the swelling technique in 0.1 M ammonium phosphate. Energized or non-energized mitochondria were preincubated with different thiol reagents and evidence is given that with a slow-reacting thiol reagent, ethacrynate, the inactivation of the phosphate carrier is obtained when mitochondria are energized, while poor or no inactivation occurs when mitochondria are non-energized or preincubated with Pi. Moreover, the inactivation depends on the presence of Mg2+ and on the nature of the substrate. Some comparative essays were done using N-ethylmaleimide as a thiol reagent, but no energy-linked variation of N-ethylmaleimide inhibition on phosphate transport was obtained. Taking into account the fact that both thiol-reagents incorporation into rat liver mitochondria is sitmulated by the presence of substrate, the different behaviour of these two thiol-reagents towards Pi transport is discussed on the basis of their different reactivity with SH groups.
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