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  • Title: Functional modification of liver form-B RNA polymerase activity by a protein fraction from rats accustomed to controlled feeding schedules.
    Author: Barbiroli B, Monti MG, Moruzzi MS, Mezzetti G.
    Journal: Biochim Biophys Acta; 1977 Nov 02; 479(1):69-79. PubMed ID: 911850.
    Abstract:
    A protein fraction extracted from the liver of rats accustomed to eating during the first 8 h of a daily 12 h dark period is able to increase homologous form-B RNA polymerase activity. When the protein factor is added to RNA polymerase extracted at the end of the daily fasted period (0.9:OOh) the sensitivity of enzyme to ionic strength is modified, and resembles that of the enzyme extracted during the eating period (15:00 h) either using liver deproteinized DNA or chromatin as template. The chromatin-bound RNA polymerase activity is also modified in the presence of the factor, showing a modification of enzyme sensitivity to ionic strength similar to the one induced by food intake.
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