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  • Title: Comparison of beta-thromboglobulin, flow cytometry, and platelet aggregometry to study platelet activation.
    Author: Matzdorff AC, Kemkes-Matthes B, Voss R, Pralle H.
    Journal: Haemostasis; 1996; 26(2):98-106. PubMed ID: 9119289.
    Abstract:
    This study compares granule membrane protein (GMP)-140 expression measured by flow cytometry, release of beta-thromboglobulin (beta-TG), and platelet aggregometry as markers of platelet activation in vitro. Whole blood was activated with different concentrations of thrombin. There was a significant increase in beta-TG plasma levels after stimulation with 0.01 and 0.04 U thrombin/ml. There was also an increase in GMP-140 expression, but interindividual variability was high. Aggregometry of platelet-rich plasma did not detect platelet activation and formation of platelet aggregates with 0.05 and 0.1 U thrombin/ml, while flow cytometry showed an early and significant increase of GMP-140 expression with these doses. Beta-TG release is a more sensitive marker of platelet activation than GMP-140 while flow cytometry is easier to perform and less susceptible to artifacts.
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