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  • Title: Adaptive regulation of MDCK cell taurine transporter (pNCT) mRNA: transcription of pNCT gene is regulated by external taurine concentration.
    Author: Han X, Budreau AM, Chesney RW.
    Journal: Biochim Biophys Acta; 1997 Apr 10; 1351(3):296-304. PubMed ID: 9130593.
    Abstract:
    NaCl-dependent taurine transporter (pNCT) activity of MDCK cells (Madin-Darby canine kidney) is up- or down-regulated by medium taurine manipulation. In this study we found that the abundance of pNCT mRNA was up- or down-regulated after cells were incubated in media containing 0 microM taurine or 500 microM taurine for 24 h. Down-regulation was observed after 12 h exposure to high taurine (500 microM) and mRNA abundance was appreciably reduced after 72 h exposure. Nuclear run-off assays show that the gene for pNCT is induced at the transcriptional level by taurine. Addition of cycloheximide blocked the adaptive response and reduced transcription of pNCT mRNA in MDCK cells. Cycloheximide had virtually no effect on pNCT mRNA stability, suggesting that ongoing protein synthesis is required for adaptive regulation of pNCT gene transcription.
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