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  • Title: [Comparison of 2 dyes in the detection of Pneumocystis carinii].
    Author: Casanova-Cardiel LJ, Cedillo-Rivera R, Garduño-Rodríguez G, Muñoz O.
    Journal: Rev Invest Clin; 1996; 48(6):443-7. PubMed ID: 9132888.
    Abstract:
    OBJECTIVE: To evaluate two staining procedures used in the detection of P. carinii. MATERIAL AND METHODS: Bronchoalveolar lavage samples were obtained from 20 immunosupressed rats. The samples were stained by three procedures: o-toluidine blue (OTB), Diff Quik (DQ), and a silver stain as the gold standard. The microscopic search for P. carinii was made independently by two observers. RESULTS: Fifteen of the 20 samples were positive to P. carinii with the silver stain. The sensitivity with DQ was 27% and the specificity 100%. With OTB the sensitivity was 93% and the specificity was 80%. The inter-raters' agreement by kappa was 0.11 for DQ and 0.53 for OTB. CONCLUSION: Our results suggest that the silver stain should be used for an adequate diagnosis of pneumonia caused by P. carinii.
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