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  • Title: [Chemiluminescence of peripheral blood T and B lymphocytes in patients with burn leukoma after lamellar keratoplasty].
    Author: Travkin AG, Derevianko VP.
    Journal: Vestn Oftalmol; 1997; 113(1):25-7. PubMed ID: 9133039.
    Abstract:
    Lamellar keratoplasty was carried out in 50 patients after eye burns. The following methods were used in the study: assessment of the intensity of chemiluminescence, electron microscopy, and assessment of T-lymphocyte subpopulations by cytofluorograph (USA) and OCT monoclonal antibodies (USA). Free-radical activation was detected by chemiluminescence during the first hours of keratoplasty in the recipient's peripheral blood leukolymphocytic suspension, whereas immunocorrecting lymphocytes did not change during this period. On days 1-2 statistically reliable changes occurred in the immune system. By day 5 electron microscopy of the leukolymphocytic suspension showed cooperation of immunocompetent cells. The course of the corneal transplant taking in was for 2 years associated with the T-helper to T-suppressor imbalance, increase of chemiluminescence level, and detection of immune lymphocytes and their cooperation under electron microscope. The authors claim that primary formed peroxides act as nonspecific participants of metabolism by altering the functional activity of immunocompetent cells; hence, a new trend in the study of cell-mediated immunity appears: immunobiophysical.
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