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  • Title: Identification of two genetic markers that distinguish pathogenic and nonpathogenic strains of Acanthamoeba spp.
    Author: Howe DK, Vodkin MH, Novak RJ, Visvesvara G, McLaughlin GL.
    Journal: Parasitol Res; 1997; 83(4):345-8. PubMed ID: 9134555.
    Abstract:
    Species-level identification of Acanthamoeba isolates is difficult and gives little or no indication of the isolate's pathogenicity. We identified two amplification-based genetic markers that were highly correlated with pathogenicity in Acanthamoeba spp. One marker, designed to amplify a 485-bp fragment of the small-subunit ribosomal RNA gene (ssrDNA), was preferentially amplified from the nonpathogenic strains; amplifications from the pathogenic strains yielded anomalous fragments of 650 and 900 bp. A second marker was developed on the basis of the anomalous 650-bp fragment. Primers to this sequence preferentially amplified a noncoding locus (called Ac6) only from the pathogenic strains. These two genetic markers may be useful for identification of pathogenic Acanthamoeba spp. strains.
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