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  • Title: Cytotoxic effect of cytokines on murine colon carcinoma cells involves TNF-mediated apoptosis.
    Author: Sveinbjørnsson B, Rushfeldt C, Olsen R, Smedsrød B, Seljelid R.
    Journal: Biochem Biophys Res Commun; 1997 Apr 07; 233(1):270-5. PubMed ID: 9144436.
    Abstract:
    We have studied the cytotoxic effect of TNF-alpha on C-26 murine colon carcinoma cells in vitro. Treatment with TNF-alpha alone did not result in any demonstrable cytotoxicity. However, when combined with IFN-gamma, the cytotoxic effect of TNF-alpha was enhanced in a dose-dependent manner. An agonistic TNF-R1 specific antibody and recombinant human TNF-alpha both exerted a cytotoxic effect when combined with IFN-gamma, suggesting that the cytotoxicity was mediated through the TNF-R1. The cytotoxicity was associated with production of nitric oxide without any direct involvement in the cytotoxic effect. At the ultrastructural level, treated cells displayed a typical apoptotic morphology which was not accompanied by internucleosomal cleavage of DNA as shown by conventional electrophoresis.
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