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  • Title: Purification to homogeneity of spinach nitrite reductase by ferredoxin-sepharose affinity chromatography.
    Author: Ida S.
    Journal: J Biochem; 1977 Sep; 82(3):915-8. PubMed ID: 914814.
    Abstract:
    Assimilatory nitrite reductase was purified 1,700-fold with a yield of 22% from spinach leaves with a procedure involving ammonium sulfate fractionation, DEAE-cellulose and DEAE-Sephadex chromatography, gel filtration and ferredoxin-Sepharose affinity chromatography. The purified enzyme was apparently homogeneous as shown by disc and SDS-gel electrophoresis with a specific activity (mumol NO2-reduced/min/mg protein) of 140.
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