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  • Title: Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
    Author: Entrican G, Deane D, MacLean M, Inglis L, Thomson J, McInnes C, Haig DM.
    Journal: Vet Immunol Immunopathol; 1996 Mar; 50(1-2):105-15. PubMed ID: 9157676.
    Abstract:
    Recombinant ovine granulocyte/macrophage colony-stimulating factor (rOv GM-CSF) has been expressed in Chinese hamster ovary cells. A stable, cloned line of these cells has been established which secretes high levels (40 mu g ml(-1)) of rOv GM-CSF. Three murine monoclonal antibodies (mAbs) were produced which reacted with rOv GM-CSF on Western blots. These mAbs also neutralised the activity of both recombinant and native Ov GM-CSF in a bone marrow haemopoietic progenitor cell assay. Two of the mAbs, which recognise mutually exclusive epitopes, were selected for the development of a sandwich enzyme-linked immunosorbent assay (ELISA) to measure GM-CSF in biological samples of ovine origin.
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