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  • Title: Artificial insemination and in-vitro fertilization using donor spermatozoa: a report on 15 years of experience.
    Author: Clarke GN, Bourne H, Hill P, Johnston WI, Speirs A, McBain JC, Baker HW.
    Journal: Hum Reprod; 1997 Apr; 12(4):722-6. PubMed ID: 9159432.
    Abstract:
    Donor insemination (DI) using cryopreserved semen commenced at The Royal Women's Hospital in 1976. Over the next 15 years we performed 5953 treatment cycles to achieve 816 pregnancies (13.7% per cycle) and 706 live births. In-vitro fertilization (IVF) using donor spermatozoa commenced in 1986. Over the next 5 years we performed 303 treatment cycles for 185 couples. Including subsequent transfer of cryopreserved embryos, a total of 33% of couples achieved a successful pregnancy by IVF. Statistical analysis indicated that, for DI pregnancies, the most important semen variable was the percentage post-thaw motility, whilst for normal fertilization in IVF it was the pre-freeze motility. These results may be explained by the compensatory effects of post-thaw processing of spermatozoa for IVF, but not for DI in our clinic.
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