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  • Title: [Glutamate deamination and transamination in the presence of dinitrophenol].
    Author: Nilova NS.
    Journal: Ukr Biokhim Zh; 1977; 49(5):23-6. PubMed ID: 919052.
    Abstract:
    Oxidation of glutamate by the brain mitrochondrial fraction was studied in the in vitro experiments in the presence of 2,4-DNP in concentrations of 5.10(-6) and 1.10(-3) M. 5.10(-6) M DNP is shown to stimulate ammonia and aspartate formation when glutamate in a concentration of 10 mM was used. Under these conditions an increase in O2 consumption occurred. When 1.10(-3) DNP was used, O2 consumption was inhbited. In this case ammonia formation was more intensive and simultaneously aspartate production was decreased. An increase in the glutamate concentration up to 20 mM diminished 2,4-DNP effects on ammonia and aspartate production.
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