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  • Title: Simultaneous identification and quantitation of codeine, morphine, hydrocodone, and hydromorphone in urine as trimethylsilyl and oxime derivatives by gas chromatography-mass spectrometry.
    Author: Broussard LA, Presley LC, Pittman T, Clouette R, Wimbish GH.
    Journal: Clin Chem; 1997 Jun; 43(6 Pt 1):1029-32. PubMed ID: 9191557.
    Abstract:
    Following enzymatic hydrolysis of urine, a gas chromatography-mass spectrometry method for the simultaneous determination of codeine, morphine, hydrocodone, and hydromorphone uses hydroxylamine to form oxime derivatives of the keto-opiates (i.e., hydrocodone, hydromorphone, oxycodone, and oxymorphone). These trimethylsilyl-derivatized forms no longer interfere with the detection and quantitation of codeine and morphine. Samples are extracted on solid-phase columns and quantitated by deuterated internal calibrations of each analyte with selected ion monitoring. Codeine, morphine, hydrocodone, and hydromorphone are completely separated, allowing simultaneous quantitation without interference and a chromatographic analysis time < 9 min.
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